Hi,
in order to find out whether the application reaches ASE you can enable the following two parameters:
execute sp_configure "log audit logon failure", 1
execute sp_configure "log audit logon success", 1
In the errorlog you'll find information regarding logon like this:
00:00000:00050:2015/04/08 12:37:31.29 Logon Login succeeded. User: db_admin
If you see the application login, then it was able to connect to ASE and failed afterwards.
If the application login does not appear, it does not reach ASE.
The functionality is disabled by setting the config values to 0.
execute sp_configure "log audit logon failure", 0
execute sp_configure "log audit logon success", 0
Best regards,
Juergen